Xiu-Bin Sun1,2,
Shao-Mei Wang3,
Tao Li4,
Yong-qing Yang5 
For correspondence:- Yong-qing Yang Email: yangyq336@gmail.com Tel:+8653185695114
Received: 23 November 2014 Accepted: 22 March 2015 Published: 26 April 2015
Citation: Sun X, Wang S, Li T, Yang Y. Anticancer activity of linalool terpenoid: Apoptosis induction and cell cycle arrest in prostate cancer cells. Trop J Pharm Res 2015; 14(4):619-625 doi: 10.4314/tjpr.v14i4.9
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the anticancer activity of linalool against human prostate cancer (DU145) cells.
Methods: The anticancer activity of linalool against DU145 cancer cells was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry, using propidium iodide and Annexin V-FITC, was applied to study apoptosis and cell cycle phase distribution. Inverted light microscopy was used to study the effect of linalool on cell morphology and apoptotic body formation in DU145 cells while gel electrophoresis was employed to evaluate the effect of linalool on DNA fragmentation.
Results: Linalool induced a dose-dependent as well as time-dependent growth inhibitory effect on DU145 prostate cancer cells. It induced sub-G1 phase growth arrest which led to increase in sub-G0/G1 cell population after treatment with increasing doses of linalool. DNA ladder appeared to be more evident with increasing linalool concentration. However, no DNA fragments were observed in the control groups. It was observed that 4.36, 11.54, 21.88 and 15.54 % of the cells underwent early apoptosis after treatment with 0 (no linalool treatment), 20, 40, and 80 µM of linalool, respectively. Compared to control cells, linalool treatment resulted in the appearance of cell shrinkage along with membrane blebbing which are characteristic features of cell apoptosis.
Conclusion: The findings of this study indicate that linalool can be developed as a plant-based chemotherapeutic agent against prostate cancer
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